Human induced pluripotent stem cells (iPSCs) play an important role in disease modeling and drug screening, and have tremendous potential for regenerative medicine. Over half all reported pathogenic mutations are caused by single-nucleotide polymorphisms (SNP); as an example, Miyoshi myopathy is a congenital muscle wasting disease caused by a mutation in the dysferlin (DYSF) gene. The correction of such mutations in iPSCs is critical to generating corrected isogenic clones for basic research and for autologous use in clinical applications. However, low efficiencies of correction (<5%) result in time-consuming and laborious screening of many clones to establish an iPSC line. Hotta et al, used the MaxCyte® platform to electroporate 100 bp single stranded oligodeoxynucleotides (ssODN) and CRISPR-Cas9 RNP achieving greater than 70% homozygous SNP correction in the DYSF gene of Miyoshi myopathy patient iPSCs.