Small-scale RUO Processing Assemblies
Static electroporation processing assemblies for research applications in small volume scales with both single-well and multi-well options.
Research Use Only (RUO) Processing assemblies aid the discovery and development of applications at small scales. These static electroporation consumables are made from high-grade, inert materials to protect precious cells with additional design features for easy sample loading and recovery.
Choose the right processing assembly for your application
OC-25x3TM
Small volume for limited cell number applications, multi-well
OC-25x3™
Configuration: 3-well, 25 µL maximum volume per well
Cell Range: 7.5x104 - 5x106 per well
Volume Range: 15 - 25 µL per well
Application: Research Use Only
Catalog: SOC-25x3
Graph Details: K562 cells were suspended in MaxCyte® Electroporation Buffer with pGFP at low concentration (200 µg/mL) or high concentration concentration (400 µg/mL) and transfected in replicates of 2 processing assemblies using low, medium and high electroporation energy. GFP fluorescence was assessed by flow cytometry 24 hours after electroporation.
R-50x3TM
Small volume applications and optimization, multi-well
R-50x3™
Configuration: 3-well, 55 µL maximum volume per well
Cell Range: 2.25x105 – 1.1x107 per well
Volume Range: 45 - 55 µL per well
Application: Research Use Only
Catalog: ER050U3-10
Graph Details: Primary human natural killer (NK) cells were collected from 4 individual donors and activated overnight in 1 ng/mL IL-15. The following day, 3 - 4 x 106 NK cells along with 5 μg mRNA GFP and 45 μL MaxCyte Electroporation Buffer was loaded into each well of the R-50x3 processing assembly and the OC-100 was used as the device control. Cells were electroporated on the MaxCyte GTx™ instrument, then collected and cultured in 1 ng/mL IL-15 media for 24 hours then assessed by flow cytometry. Graphs show the average results and standard error of cells taken from 4 individual donors electroporated with 5 processing assemblies, 2 used for the same donor, with each well of the R-50x3 analyzed individually. The negative control is the average of 4 samples. Experimental data courtesy of Dr. Todd Fehniger’s Laboratory, Washington University in St Louis, Department of Medicine, Division of Oncology with special appreciation to Pamela Wong.
R-50x8TM
Small volume discovery applications, multi-well strip
R-50x8™
Configuration: 8-well, 55 µL maximum volume per well
Cell Range: 2.25x105– 1.1x107 per well
Volume Range: 45 - 55 µL per well
Application: Research Use Only
Catalog: ER050U8-03
Graph Details: K562 cells at 5x107 cells/mL were electroporated with plasmid DNA encoding GFP at low energy (Opt-1) and high energy (Opt-10). Four R-50x8 PAs and eight OC-100 PAs were evaluated at each energy level for both GFP expression and cell viability 48 hours post-transfection via flow cytometry analysis. Results are an average of 16 wells for the R-50x8 and 8 wells for the OC-100 for each energy level.
OC-100TM RUO
Small volume applications and optimization, single-well
OC-100™ RUO
Configuration: Single well, 100 µL maximum volume
Cell Range: 2.5x105 – 2x107
Volume Range: 50 - 100 µL
Application: Research Use Only
Catalog: SOC-1
Graph Details: K562 cells were suspended in MaxCyte Electroporation Buffer with pGFP at low concentration (200 µg/mL) or high concentration concentration (400 µg/mL) and transfected in replicates of 2 processing assemblies using low, medium and high electroporation energy. GFP fluorescence was assessed by flow cytometry 24 hours after electroporation.
OC-100x2TM
Small volume applications and optimization, multi-well
OC-100x2™
Configuration: 2-well, 100 µL maximum volume per well
Cell Range: 2.5x105 – 2x107 per well
Volume Range: 50 - 100 µL per well
Application: Research Use Only
Catalog: SOC-1x2
Graph Details: K562 cells were suspended in MaxCyte Electroporation Buffer with pGFP into each well of 3 OC-100x2 PAs and 3 OC-100 PAs. Samples were analyzed 24 hours post-electroporation and assayed for fluorescence to measure efficiency. The average for each well is shown in the graph.
Reagents & Supporting Products
Related products to consider with the small-scale series of Processing Assemblies.
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