February 1, 2017 – EST
Learning Objectives
- Flow electroporation transfection technology enables highly efficient loading of plasmids, messenger RNA and other molecules into cell lines and primary cells at scales ranging from <1e6 to 2e11 cells
- Discuss how the high efficiency and viability allows generation of stable pools and clones with reduced time and effort
Share data on efficient targeted modification of mammalian genomes for cell therapy and protein expression using CRISPR/Cas-9 and zinc finger nucleases
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