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Topic: Development of a genome-wide CRISPR screen in CD4+ T cells to identify drug targets for immune-mediated inflammatory diseases

Description: Immune-mediated inflammatory diseases (IMIDs) are a group of disorders characterised by tissue inflammation as a result of dysregulated immune responses. This webinar will showcase the development of a genome-wide CRISPR screen in primary T cells to identify drug targets for IMIDs, and will cover key areas of workflow optimisation, including lentiviral transduction, Cas9 electroporation in bulk cell populations and fluorescence-activated cell sorting. Preliminary data obtained from a recent ‘mini screen’ will be discussed and the session will close with some key learnings from the workflow development process.

Time: Apr 7, 2021 09:30 AM in Eastern Time (US and Canada)