The International Society for Stem Cell Research (ISSCR) Annual Meeting is an annual gathering of the world's leading stem cell researchers from around the globe. This 4-day event features presentations on the latest advances in stem cell research, as well as networking opportunities and social events.
Come visit us and discover MaxCyte’s cGMP-compliant non-viral cell engineering platform and how it enables the acceleration of therapeutic development from concept to clinic.
While at the meeting, don't miss these featured talks and posters:
MicroRNA-responsive synthetic messenger RNA and its medical applications
Date: June 15th
Time: 12:00-1:00 p.m. (EST)
Location: Room 258
Presenters: Hiroki Ono, PhD, postdoctoral fellow in Center for iPS Cell Research and Application (CiRA) at Kyoto University, Japan.
Identification and purification of specific cell types are essential for cell therapy (e.g., transplantation of purified cells in regenerative medicine), as well as for basic biological research. MicroRNA (miRNA) is a class of small non-coding RNAs that regulates gene expression post-transcriptionally and can serve as a marker of specific cell types. To identify and purify specific cell types based on miRNAs, we have previously developed miRNA switch, miRNA-responsive modified RNA (modRNA) transcribed in vitro. miRNA switch has enabled the FACS-free elimination of contaminating human induced pluripotent stem cells (iPSCs) from iPSC-derived cardiomyocytes.
Although lipid-based transfection efficiently delivers modRNAs into cells, several types of cells (e.g., hematopoietic stem cells; HSCs) remain difficult to transfect. MaxCyte electroporation system efficiently delivers modRNAs into hard-to-transfect cells. Recently, we are trying to purify hematopoietic stem cells (HSCs) with miRNA switches.
In this session, we will share the recent progress in cell purification with miRNA switches for regenerative medicine.