ISCT – International Society for Cell & Gene Therapy

Expanding the Paradigm of Immuno-Gene Therapy With ground-breaking clinical success and exponentially growing research leading to FDA approved products, lymphocyte engineering is the way of the future. Join us for the 3rd International Conference on Lymphocyte Engineering – where the world’s health innovators in immuno-gene therapy come together.

Presentation title: Scalable Electroporation of Adult Keratinocytes with Multiplexed CRISPR

In the past, engineered keratinocyte-based cell therapies have been limited by the lack of efficient transfection methods for adult keratinocytes. Here we aimed to develop a GMP-compliant, scalable cell
engineering process using the MaxCyte® cell electroporation platform to transfect neonatal and adult primary keratinocytes from four distinct anatomical locations. Delivery of multiple CRISPR RNPs in a single electroporation achieved highly efficient, multiplexed gene editing in a simple, adaptable process.

These improvements in transfection efficiency and cell viability reduced keratinocyte engineering times by up to 4 weeks, with a significantly higher success rate than a standard chemical transfection method.

Lastly, we demonstrate the scalability of the MaxCyte electroporation process, enabling the engineering of millions of primary keratinocytes without any loss of efficacy.