Effectual Labeling of Natural Killer Cells with Upconverting Nanoparticles by Electroporation for In Vivo Tracking and Biodistribution Assessment
Effectual Labeling of Natural Killer Cells with Upconverting Nanoparticles by Electroporation for In Vivo Tracking and Biodistribution Assessment
Read MoreEfficient Electroporation of Neuronal Cells Using Synthetic Oligonucleotides: Identifying Duplex RNA and Antisense Oligonucleotide Activators of Human Frataxin Expression
Oligonucleotide drugs are experiencing greater success in the clinic, encouraging the initiation of new projects.
Read MoreEfficient Large Volume Lentiviral Vector Production Using Flow Electroporation
Lentiviral vectors are beginning to emerge as a viable choice for human gene therapy. Here, we describe a method that combines the convenience of a suspension cell line with a scalable, nonchemically based, and GMP-compliant transfection technique known as flow electroporation (EP).
Read MoreEfficient mRNA-based Genetic Engineering of Human NK Cells with High-Affinity CD16 and CCR7 Augments Rituximab-induced ADCC against Lymphoma and Targets NK cell Migration Towards the Lymph Node Associated Chemokine CCL19
For more than a decade, investigators have pursued methods to genetically engineer natural killer (NK) cells for use in clinical therapy against cancer.
Read MoreEfficient production of bispecific IgG of different isotypes and species of origin in single mammalian cells
Bispecific IgG production in single host cells has been a much sought-after goal to support the clinical development of these complex molecules.
Read MoreEfficient Responses in a Murine Renal Tumor Model by Electroloading Dendritic Cells With Whole-Tumor Lysate
Electroporation of dendritic cells (DCs) with tumor lysate elicited greater antitumor responses in vitro and in vivo, using less lysate than standard coincubation. Electroloaded DCs had normal surface marker expression and matured into competent antigen-presenting cells. In a renal carcinoma (RENCA) model, mice were pretreated with lysate-loaded DCs before tumor challenge.
Read MoreEfficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein
Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs)
Read MoreA Pilot Study of Autologous Tumor Lysate-Loaded Dendritic Cell Vaccination Combined With Sunitinib For Metastatic Renal Cell Carcinoma
Background Sunitinib, a tyrosine kinase inhibitor currently in use for the treatment of metastatic renal cell carcinoma (mRCC), has been reported to modulate immunosuppressive cells such as myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs) in addition to exerting anti-angiogenic effects.
Read MoreA nonviral, nonintegrating DNA nanovector platform for the safe, rapid, and persistent manufacture of recombinant T cells
The compelling need to provide adoptive cell therapy (ACT) to an increasing number of oncology patients within a meaningful therapeutic window makes the development of an efficient, fast, versatile, and safe genetic tool for creating recombinant T cells indispensable.
Read MoreA Scalable Cell-Loading System for Non-Viral Gene Delivery and Other Applications
Across many areas of biopharmaceutical development, the goal of consistently transfecting appropriate quantities of DNA into cells has often been a significant bottleneck.
Read More