Amplicon-seq™

Confirmation assay

SeQure DX's Amplicon-seq assay provides high-sensitivity confirmation of on- and off-target edits in gene-edited cell lines and tissues. Targeted amplicon sequencing is guided by the results of ONE-seq, DEUX-seq and GUIDE-seq, enabling precise validation of gene editing outcomes in your cells of interest. By delivering detailed and accurate editing confirmation, Amplicon-seq supports regulatory expectations, reduces risks of IND filing delays, and strengthens the safety profile of your cell and gene therapy development programs.

Amplicon-seq overview

SDX-Amplicon-seq Overview

Amplicon-seq

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High-sensitivity confirmation of off-targets in your cell type of interest post-editing. Informed by our nomination assays, we know exactly where we need to look to confirm editing.

Confirmation

Verifies cellular edits

  • Sensitive detection methods
  • Relevant cell types
  • On- and off-target analysis

Cellular editing assessment

IND filings

  • Biologic impact assessment
  • GLP

Featured resources

Summary table of confirmed off-target sites for the guides GENE1-1, GENE1-3 and GENE1-4.
Example Customer Report
Amplicon-seq Confirmation Assay Example Report
Breakpoint site alignment. The reference CCR5 target site is shown at the top. Mismatched nucleotides and indels are highlighted. The alignment score is listed on the right. Diamonds indicate off-target sites detected at validated inter-chromosomal translocation breakpoints.
Poster
SAFER Detection for Efficient Interrogation of DNA Rearrangements in Gene-Edited Human Cells
Circos plot showing rearrangements involving GENE1-1 that were detected in 3/3 replicates. The target chromosome is expanded to facilitate visualization of intra-chromosomal rearrangements. The weight of the lines indicating rearrangements are proportional to log10 of the average total UMI read counts.
Example Customer Report
SAFER Detection Confirmation Assay Example Report
View all resources
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Related confirmation assays

SAFER Detection

High-sensitivity confirmation of structural rearrangements in your cell type of interest post-editing. NGS interrogation allows for resolution down to exact genomic coordinates. 

Learn more about our comprehensive suite of assays spanning from early discovery through pre-clinical and clinical development.

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