SeQure Dx on-target and off-target editing assessment services SeQure Dx comprehensive suite of on-target and off-target assessment assays

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Comprehensive suite of on-target and off-target assessment assays

Our comprehensive off-target gene editing assessment platform integrates screening, nomination, and confirmation assays, providing unmatched precision in guide RNA selection and off-target risk assessment. By addressing safety and regulatory expectations early, our platform reduces development risks, streamlines therapeutic design, and accelerates your path to the clinic with confidence and efficiency.

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A Comprehensive Portfolio of Gene Editing Assays

Summary-table
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Screening Assays

Preview of off-target risk for informed guide selection

Guide Profiler™

In silico preview of potential off-target effects associated with your gene editing tool. Enables you to start your gene editing risk assessment by rapidly screening sgRNAs to understanding on-target efficacy and off-target editing risks.

Guide Select™

Off-target assessment assay designed for screening multiple guide RNAs in parallel. Variant aware searching within a limited search space enables high-powered, low-cost risk assessment for guide RNA selection.

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Nomination Assays

Comprehensive off-target assessment

ONE-seq®

Gold-standard, population-scale, variant-aware, off-target detection. Candidate off-target enumeration, enrichment, and biochemical in vitro cutting combined with deep sequencing enables high sensitivity detection of low frequency off-target events. 

DEUX-seq™

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased biochemical off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts. 

GUIDE-seq™

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased cell-based off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.  

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Confirmation Assays

Verifies cellular edits and structural rearrangements

Amplicon-seq™

High sensitivity confirmation of off-target effects in your cell type of interest post-editing. Informed by nomination assays, NGS pinpoints off-target edits with resolution down to exact genomic coordinates.

SAFER Detection™

High sensitivity confirmation of structural rearrangements in your cell type of interest post-editing. NGS interrogation allows for resolution down to exact genomic coordinates. 

Resources

ONE-seq for Variant-Aware Therapeutic Guide Selection-Off target proximity to target site
Poster
ONE-seq for Variant-Aware Therapeutic Guide Selection
SAFER Detection for Efficient Interrogation of DNA Rearrangements in Gene-Edited Human Cells-Figure 2D
Poster
SAFER Detection for Efficient Interrogation of DNA Rearrangements in Gene-Edited Human Cells
SDX-Guide Profiler-Poster-Figure 1G
Poster
Guide Profiler: A Genetic Variant-Aware Computational Tool for Improved Guide RNA Selection for CRISPR-based Therapeutic Applications
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