SeQure DX On-Target and Off-Target Editing Assessment Services SeQure DX Comprehensive Suite of On-target and Off-target Assessment Assays SeQure DX SAFER Detection – Confirmation Assay

SAFER Detection™

Confirmation assay

SeQure DX's SAFER Detection assay confirms on-target, off-target and homology-mediated structural rearrangements resulting from gene editing. It also profiles integration sites and determines vector copy number following viral transduction. Our comprehensive biological impact analysis report addresses FDA expectations for structural rearrangement data, helping to mitigate risks of IND filing delays and enhancing the safety profile of your cell and gene therapy development programs.

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SAFER Detection overview

SDX-SAFER Detection Overview

SAFER Detection​

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High-sensitivity confirmation of structural rearrangements in your cell type of interest post-editing. NGS interrogation allows for resolution down to exact genomic coordinates. Additionally capable of detecting gene insertions from viral-mediated cell engineering workflows.

Confirmation

Verifies structural rearrangements

  • Sensitive detection ​
  • Relevant cell types​
  • On-target, off-target and homology-mediated rearrangements

Cellular editing assessment

IND filings

  • Biologic impact assessment​
  • GLP

Featured resources

Breakpoint site alignment. The reference CCR5 target site is shown at the top. Mismatched nucleotides and indels are highlighted. The alignment score is listed on the right. Diamonds indicate off-target sites detected at validated inter-chromosomal translocation breakpoints.
Poster
SAFER Detection for Efficient Interrogation of DNA Rearrangements in Gene-Edited Human Cells
Circos plot showing rearrangements involving GENE1-1 that were detected in 3/3 replicates. The target chromosome is expanded to facilitate visualization of intra-chromosomal rearrangements. The weight of the lines indicating rearrangements are proportional to log10 of the average total UMI read counts.
Example Customer Report
SAFER Detection Confirmation Assay Example Report
A map of the world with different colored dots representing the varying number of samples across different geographic regions.
Poster
ONE-seq for Variant-Aware Therapeutic Guide Selection
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Related confirmation assay

Amplicon-seq

High-sensitivity confirmation of off-targets in your cell type of interest post-editing. Informed by our nomination assays, we know exactly where we need to look to confirm editing.

Learn more about our comprehensive suite of assays spanning from early discovery through pre-clinical and clinical development.

View SeQure DX Comprehensive Suite of On-Target and Off-Target Assessment Assays
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