Sterile Well Processing Assembly

  • Classic design, proven performance
  • Multi-well and single well options to fit a variety of applications
  • Made of high grade and inert materials to protect precious cells
  • Well designs allow for efficient cell loading and recovery

OC RUO PAs work with ExPERT STx and ATx, STX and ATX Instruments

Explore OC PAs:

First Sterile Well Processing Assembly • Small volume for limited cell number applications
• Multi-well for increased throughput; lower cost per transfection
• V-shaped well designs for efficient cell recovery
 Configuration: 3-well, 25 µL volume per well
Cell Range: 75K to 5 million cells per well
Volume Range: 15 µL - 25 µL per well working volume
Use: Research Use Only
 Catalog Number: SOC-25X3 Contents: 10 PAs + 100 mL EP Buffer K562 cells were suspended in MaxCyte electroporation bu erK562 cells were suspended in MaxCyte electroporation buffer with pGFP with pGFP at low at low concentration (200 ug/mL) or high concentrationconcentration (200 ug/mL) or high concentration (400 ug/mL) and transfected in replicates of 2 (400 ug/mL) and transfected in replicates of 2 processing processing assemblies using low, medium and high electroporation energy.assemblies using low, medium and high electroporation energy. GFP Fluorescence were assessed by GFP Fluorescence were assessed by 􀀂ow cytometry 24 hours􀀂ow cytometry 24 hours after electroporation.after electroporation. First Sterile Well Processing Assembly • Small volume applications and experimental optimization
• For quick single sample transfection
 Configuration: Configuration: Single well, 100 uL volume
Cell Range: 500K to 20 million cells
Volume Range: 50 µL - 100 µL working volume
Use: Research Use Only
 K562 cells were suspended in MaxCyte
electroporation buffer with pGFP at low
concentration (200 ug/mL) or high concentration
(400 ug/mL) and transfected in replicates of 2
processing assemblies using low, medium
and high electroporation energy. GFP
Fluorescence were assessed by flow cytometry
24 hours after electroporation. Catalog Number: SOC-1 Contents: 10 OC-100 PAs + 100 mL EP Buffer First Sterile Well Processing Assembly • Multi-well for increased throughput; lower cost per transfection
• V-shaped well design for efficient cell recovery
 Configuration: 2-well, 100 µL volume per well
Cell Range: 500K to 20 million cells per well
Volume Range: 50 µL - 100 µL per well working volume
Use: Research Use Only
 Catalog Number: SOC-1x2 Contents: 10 OC-100x2 PAs + 100 mL EP Buffer Graph Details: K562 cells were suspended in MaxCyte electroporation buffer with pGFP into each well of 3 OC-100x2 PAs and 3 OC-100 PAs. Samples were analyzed 24 hours post-electroporation and assayed for fluorescence on a flow cytometer to measure efficiency. Average result for each well is shown in the graph. First Sterile Well Processing Assembly • Mid-scale applications and scale-up optimization
• For quick single sample transfection
 Configuration: Single well, 400 µL volume
Cell Range: 1 million to 80 million cells
Volume Range: 200 µL - 400 µL working volume
Use: Research Use Only
 Catalog Number: SOC-4 Contents: 10 OC-400 RUO PAs + 100 mL EP Buffer Graph Details: HEK 293 cells were suspended in MaxCyte Electroporation Buffer at a density of 1e8/mL with pGFP at 200 ug/mL to make a master mix. 400 uL of master mix was loaded into the OC-400, 1 mL was loaded into one CL-1.1 and 3.5 mL was loaded into a second CL-1.1. Transfection was performed using the MaxCyte HEK electroporation protocol for all 3 PAs. Cells were plated at 3 different plating densities post-transfection and then were analyzed for expression on a flow cytometer after 48 hours.
 OC-100 OC-25x3 OC-100x2 OC-400