TCR-T Cell Therapy

Whether you want to express exogenous T cell receptor (TCR) mRNA or to knock out endogenous TCR with CRISPR RNP, MaxCyte® ExPERTTM instruments can streamline your journey to success with reliable, high-efficiency electroporation and high cell viability.

Explore multiplex engineering

Designing potent, safe and persistent T cell therapies requires sophisticated engineering strategies, which can be challenging to implement, particularly at the GMP scale necessary for the clinic. Explore how MaxCyte enabled the first-ever human clinical trial of multiplex CRISPR gene-edited T cell therapy.


CT Scans show 50% tumor regression after infusion with TCR immunotherapy.

Adapted from Carl June Science, 2020, 367(6481):eaba7365.


TCR expression was achieved in 84-93% of T cells.

This content was reproduced with the kind permission of Lion TCR.

Express exogenous TCR mRNA

Engineering T cells to express new TCRs can be challenging. Timelines, cost and potential toxicity are all factors that must be balanced when designing new cell therapies. MaxCyte electroporation offers a high-efficiency, scalable, cost-effective and safe approach to delivering a variety of gene editing tools, including exogenous mRNA for optimized T cell manufacturing.

Generating TCR-T cells is rapid, scalable and clinically feasible

Steer your way to a successful T cell therapy by partnering with MaxCyte. For sophisticated multiplexed engineering and highly efficient delivery of a variety of payloads, we are here to support your discovery from concept to commercialization.

Research applications

Gene Editing

Navigate the complexities of genome engineering with highly efficient delivery.

Cell Therapies

Discover scalable engineering to enable safe and potent cell therapies.

Related Resources

Non-Viral Engineering of Human iPSCs to Manufacture Rejuvenated TCR T Cells for Cancer Immunotherapy.
Lion-TCR-1-1200x200 (2)
Efficient and Scalable T Cell Engineering for Advancing Cancer Immunotherapy of Hepatocellular Carcinoma by a cGmp-Compliant Non-Viral Cell Engineering Platform.
Scientific Brief
Multiplex CRISPR-mediated Gene Editing Towards the Creation of a Universal CAR T Cell.
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Electroporation Systems

Supported by numerous publications and clinical trials, our ExPERT GTx® instrument is the next generation of the industry’s leading, scalable electroporation technology for complex cell engineering.

The GTxTM is capable of high-performance delivery of virtually any molecule, into any cell, at any scale with the unique ability to transfect primary cells, stem cells and cell lines with high cell viability and transfection efficiencies routinely over 90%.


Reagents and Processing Assemblies

MaxCyte’s consumable products provide users with a variety of options for project scale and throughput from discovery through cGMP manufacturing using a single platform. Our range of Processing Assemblies allows users to transfect a variety of cell sample volumes to meet specific application needs. MaxCyte’s Electroporation Buffer is animal-derived component free and safe for all cell types ensuring consistent, high-performance transfection.

Ready to learn how to advance your T cell engineering projects?

Our experienced team is here to help.