
Figure 1. A manufacturing protocol was developed to allow the generation of clinical-grade recombinant T cells using nS/MARt vectors. For this, the CliniMACS Prodigy® device (Miltenyi®), a fully automated and closed system for the isolation and culturing of primary human CD3+ cells was coupled with the ExPERT GTx™ large scale electroporation platform (MaxCyte®).

Figure 2. A) CD3+ cells were isolated from a leukapheresis product with the TCT process using the CliniMACS Prodigy®. B) Sorted cells were activated for 3 days with T cell TransActTM, IL-7, and IL-15. On day three 1 x 10 8 cells were counted and electroporated with 125 ug/ml of DNA using the GTxTM. Shortly after electroporation, the cells were returned to the CliniMACS Prodigy and fed with IL-7- and IL-15 supplemented medium for one day. On day 5, cells were harvested and analyzed by FACS for CAR expression. C) The capability for killing tumor cells was tested in an in vitro killing assay D) and INF-γ production measured.

Figure 3. A) FACS plots show the same percentage of CD8+ and CD4+ cells within the CD45+/CD3+ subset before and after manufacturing. B) Samples taken from the CliniMACS Prodigy® at different time points pre and post electroporation show the T cells’ proliferative capacity.
Summary
• MaxCyte Flow Electroporation® provides efficient, scalable and GMP-compliant delivery of CARs
• MaxCyte® enabled efficient delivery of nS/MARt vectors to difficult-to-transfect primary T cells
• T cell proliferation was not impaired and CD4:CD8 ratios were not altered
• Active T cells demonstrated highly efficient killing capabilities in vitro