ONE-seq
Nomination assay
SeQure's ONE-seq is a nomination assay for comprehensive identification of all putative on- and off-target effects for your gene editing tool of interest. ONE-seq leverages variant-aware off-target enumeration coupled with in vitro cutting and deep sequencing to enable precise identification of on- and off-target edits across distinct genetic sub-populations. This high-powered assay offers the most comprehensive risk assessment available for gene editing, ensuring unparalleled confidence in your therapeutic development.
ONE-seq overview
ONE-seq & ONE-Seq-BE
ONE-seq and ONE-seq-BE allow population-scale, variant-aware, off-target nomination. Candidate off-target enumeration, enrichment, and in vitro cutting combined with NGS enables high sensitivity detection of low frequency off-target events.
Comprehensive off-target assessment
- Highly sensitive
- Population-scale variant assessment
Off-target assessment
Guide RNA selection
IND filings
- Multiple orthogonal methods
- Variant effect prediction
Related nomination assays
From early discovery through pre-clinical and clinical development
