Multiple Injections of Electroporated Autologous T Cells Expressing a Chimeric Antigen Receptor Mediate Regression of Human Disseminated Tumor
Redirecting T lymphocyte antigen specificity by gene transfer can provide large numbers of tumor-reactive T lymphocytes for adoptive immunotherapy. However, safety concerns associated with viral vector production have limited clinical application of T cells expressing chimeric antigen receptors (CAR). T lymphocytes can be gene modified by RNA electroporation without integration-associated safety concerns.
Read MoreNext-generation DNA vectors: is the nS/MARt platform a viable alternative to viruses for autologous T-cell immunotherapy?
Next-generation non-viral RNA and DNA vectors have emerged as an attractive alternative for introducing CARs or TCRs into immune cells; while maintaining a high efficiency of delivery, they are more versatile and are simpler and quicker to manufacture at scale, thus increasing the number of patients who can be treated while significantly reducing the vein-to-vein time of the treatment process.
Read MoreLentivirus and AAV Production in Suspension and Adherent Cells with MaxCyte Flow Electroporation
Viral Vectors have been a core component of gene therapy since the 1980s. Since that time vector production technologies have not changed a great deal, leaving industry and academia alike to face the same challenges.
Read MorePotent immunomodulatory effects of an anti-CEA-IL-2 immunocytokine on tumor therapy and effects of stereotactic radiation
While anti-CEA antibodies have no direct effect on CEA-positive tumors, they can be used to direct potent antitumor effects as an antibody-IL-2 fusion protein (immunocytokine, ICK), and at the same time reduce the toxicity of IL-2 as a single agent.
Read MorePreclinical evaluation for engraftment of CD34+ cells gene-edited at the sickle cell disease locus in xenograft mouse and non-human primate models
Sickle cell disease (SCD) is caused by a point mutation in the b-globin gene and can be cured by the replacement of hematopoietic stem cells (HSCs).
Read MoreKeys to Successful Cell-Based Assay Development with Scalable Electroporation
Drug discovery and development is a costly, time-consuming process with a high risk of failure. One approach to save time and mitigate risk is to increase the use of cell-based assays as an alternative to biochemical assays. Cell-based assays (2D and 3D) enable interrogation of a target in a physiological context and have the potential to be used in all phases from target discovery up to preclinical development.
Read MoreOptimization of CHO Transient Gene Expression (TGE) for Multi-Gram Level Antibody Production: Effects of Expression Construct, Post Transfection Cell Density and Feed Conditions
A variety of CHO cell transient transfection methods have been reported including systems based on engineered CHO cells, unique expression systems and specialized transfection reagents, but they each have varying levels of reproducibility, scalability, and cost effectiveness and generally produce antibody titers from 10 – 100mg/L (1-5).
Read MoreOptimization of Transient Transfection of CHO cells. A comparison between transfection efficiency utilizing PEI vs. an electroporation transient transfection system
Transient gene expression in CHO cells is a difficult enterprise to obtain high titers of recombinant antibody proteins. Conducting a transient transfection in the same host as our stable cell lines allows similar post translational modification of the expressed protein or antibodies, such as glycosylation patterns.
Read MoreRapid and efficient nonviral gene delivery of CD154 to primary chronic lymphocytic leukemia cells
Interactions between CD40 and CD40 ligand (CD154) are essential in the regulation of both humoral and cellular immune responses. Forced expression of human CD154 in B chronic lymphocytic leukemia (B-CLL) cells can upregulate costimulatory and adhesion molecules and restore antigen-presenting capacity.
Read MoreRapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry
Here, we describe a novel application of biolayer interferometry for the rapid detection of antigen-specific antibody levels in plasma samples, and demonstrate its utility for quantification of SARS-CoV-2 antibodies.
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