Mid-scale RUO Processing Assemblies
Static electroporation processing assemblies for research applications in mid-volume scales.
Research Use Only (RUO) Processing Assemblies enable the discovery and development of applications at mid-scale. These static electroporation consumables are made from high-grade, inert materials to protect precious cells with additional design features for easy sample loading and recovery.
Choose the right processing assembly for your application
OC-400TM RUO
Mid-scale applications and scale-up optimization, single-well
OC-400 RUOâ„¢
Configuration: Single well, 400 µL maximum volume
Cell Range: 1x106 – 8x107
Volume Range: 200 - 400 µL
Application: Research Use Only
Catalog: SOC-4
Graph Details: To make a master mix, HEK 293 cells were suspended in MaxCyte® Electroporation Buffer at a density of 1x108 cells/mL with pGFP at 200 µg/mL. 400 µL of the master mix was loaded into the OC-400, 1 mL was loaded into one CL-1.1 and 3.5 mL was loaded into a second CL-1.1. Transfection was performed using the MaxCyte HEK electroporation protocol for all 3 PAs. Cells were plated at 3 different densities post-transfection and were analyzed for expression on a flow cytometer after 48 hours.
R-1000TM
Mid-scale applications and scale-up optimization, single-well
R-1000â„¢
Configuration: Single-well, 1000 µL maximum volume
Cell Range: 2x106 – 2x108
Volume Range: 400 µL - 1 mL
Application: Research Use Only
Catalog: ER001M1-10
Graph Details: KG1a cells were suspended in MaxCyte Electroporation Buffer and mRNA GFP. Electroporation was performed on the MaxCyte GTx™ instrument using the same protocol for all processing assemblies. Final volumes for R-1000, OC-400 and CL-1.1 were 900 μL, 400 μL and 1 mL, respectively. Nine replicates were performed for the R-1000 and OC-400 and three replicates for the CL-1.1, with averages calculated for all graphs.
CL-1.1TM RUO
Mid-scale applications and scale-up, closed chamber
CL-1.1â„¢ RUO
Configuration: Closed chamber, 3.5 mL maximum volume
Cell Range: 5x106 – 7x108
Volume Range: 1 – 3.5 mL
Application: Research Use Only
Catalog: SCL-1
Graph Details: To make a master mix, HEK 293 cells were suspended in MaxCyte Electroporation Buffer at a density of 1x108 cells/mL with pGFP at 200 µg/mL. 400 µL of the master mix was loaded into the OC-400, 1 mL was loaded into one CL-1.1 and 3.5 mL was loaded into a second CL-1.1. Transfection was performed using the MaxCyte HEK electroporation protocol for all 3 PAs. Cells were plated at 3 different densities post-transfection and were analyzed for fluorescence after 48 hours.
Related Reagents and Supporting Products
Related products to consider using with the mid-scale series of Processing Assemblies.
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