Small-scale RUO Processing Assemblies

Research Use Only (RUO) Processing assemblies aid the discovery and development of applications at small scales. These static electroporation consumables are made from high-grade, inert materials to protect precious cells with additional design features for easy sample loading and recovery.

Choose the right processing assembly for your application

R-50x96™

Small-scale Static Electroporation

R-50x96

Standard 96-well plate layout designed for high-throughput electroporation

Detachable 8-well strip format for experimental flexibility and reduced waste

Compatible with 20–50 µL process volumes for small-scale screening applications

Configuration: Standard 96 well plate, 8 well strips
Cell Range: 1x10⁵ – 1x10⁷
Volume Range: 20 - 50 µL
Application: Research use only
Catalog: DR050U96

Graph Details: The reproducibility of electroporation with the R-50x96 was demonstrated by loading FITC-dextran into Jurkat cells. Transfection efficiency was assessed in 30 replicates at both low (20 μL per well) and high (50 μL per well) sample volumes. The percentage of FITC-positive cells was determined on day 1 and day 2 post electroporation. Error bars show +/- SEM.

DTx

A new standard in high-throughput electroporation. Customizable, high-viability electroporation in a 96-well format.

R-50x3^TM

Small volume applications and optimization, multi-well

R-50x3™

Research & development applications

Efficient 3 sample processing per transfection

Easy cell recovery with V-shaped well design

Configuration: 3-well, 55 µL maximum volume per well
Cell Range: 2.25x10⁵ – 1.1x10⁷per well
Volume Range: 45 - 55 µL per well
Application: Research Use Only
Catalog: ER050U3-10

Graph Details: Primary human natural killer (NK) cells were collected from 4 individual donors and activated overnight in 1 ng/mL IL-15. The following day, 3 - 4 x 10⁶ NK cells along with 5 μg mRNA GFP and 45 μL MaxCyte Electroporation Buffer was loaded into each well of the R-50x3 processing assembly and the OC-100 was used as the device control. Cells were electroporated on the MaxCyte GTx™ instrument, then collected and cultured in 1 ng/mL IL-15 media for 24 hours then assessed by flow cytometry. Graphs show the average results and standard error of cells taken from 4 individual donors electroporated with 5 processing assemblies, 2 used for the same donor, with each well of the R-50x3 analyzed individually. The negative control is the average of 4 samples. Experimental data courtesy of Dr. Todd Fehniger’s Laboratory, Washington University in St Louis, Department of Medicine, Division of Oncology with special appreciation to Pamela Wong.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

R-50x8^TM

Small volume discovery applications, multi-well strip

R-50x8™

8-wells for increased throughput; lower cost per transfection

U-shaped well design for efficient cell loading and recovery

Sample tracking with A-H lettering and front writing space

Configuration: 8-well, 55 µL maximum volume per well
Cell Range: 2.25x10⁵– 1.1x10⁷ per well
Volume Range: 45 - 55 µL per well
Application: Research Use Only
Catalog: ER050U8-03

Graph Details: K562 cells at 5x10⁷ cells/mL were electroporated with plasmid DNA encoding GFP at low energy (Opt-1) and high energy (Opt-10). Four R-50x8 PAs and eight OC-100 PAs were evaluated at each energy level for both GFP expression and cell viability 48 hours post-transfection via flow cytometry analysis. Results are an average of 16 wells for the R-50x8 and 8 wells for the OC-100 for each energy level.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

OC-25x3^TM

Small volume for limited cell number applications, multi-well

OC-25x3™

Small volume for limited cell number applications

Multi-well for increased throughput; lower cost per transfection

V-shaped well design for efficient cell recovery

Configuration: 3-well, 25 µL maximum volume per well
Cell Range: 1x10⁵ - 5x10⁶ per well
Volume Range: 20 - 25 µL per well
Application: Research Use Only
Catalog: SOC-25x3

Graph Details: K562 cells were suspended in MaxCyte^® Electroporation Buffer with pGFP at low concentration (200 µg/mL) or high concentration concentration (400 µg/mL) and transfected in replicates of 2 processing assemblies using low, medium and high electroporation energy. GFP fluorescence was assessed by flow cytometry 24 hours after electroporation.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

OC-100^TM RUO

Small volume applications and optimization, single-well

OC-100™ RUO

Small volume applications and experimental optimization

For quick single sample transfection

Well designed for efficient cell loading and recovery

Configuration: Single well, 100 µL maximum volume
Cell Range: 2.5x10⁵ – 2x10⁷
Volume Range: 50 - 100 µL
Application: Research Use Only
Catalog: SOC-1

Graph Details: K562 cells were suspended in MaxCyte Electroporation Buffer with pGFP at low concentration (200 µg/mL) or high concentration concentration (400 µg/mL) and transfected in replicates of 2 processing assemblies using low, medium and high electroporation energy. GFP fluorescence was assessed by flow cytometry 24 hours after electroporation.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

OC-100x2^TM

Small volume applications and optimization, multi-well

OC-100x2™

Multi-well for increased throughput; lower cost per transfection

V-shaped well design for efficient cell recovery

Configuration: 2-well, 100 µL maximum volume per well
Cell Range: 2.5x10⁵ – 2x10⁷ per well
Volume Range: 50 - 100 µL per well
Application: Research Use Only
Catalog: SOC-1x2

Graph Details: K562 cells were suspended in MaxCyte Electroporation Buffer with pGFP into each well of 3 OC-100x2 PAs and 3 OC-100 PAs. Samples were analyzed 24 hours post-electroporation and assayed for fluorescence to measure efficiency. The average for each well is shown in the graph.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

*Cell numbers are approximate based on MaxCyte’s standard cell concentrations used at maximum and minimum volumes per reaction. Cell type and delivery agent will determine the optimal cell number recommended per each electroporation reaction.