Small-scale RUO Processing Assemblies

Research Use Only (RUO) Processing assemblies aid the discovery and development of applications at small scales. These static electroporation consumables are made from high-grade, inert materials to protect precious cells with additional design features for easy sample loading and recovery.

Choose the right processing assembly for your application

OC-25x3^TM

Small volume for limited cell number applications, multi-well

OC-25x3™

Small volume for limited cell number applications

Multi-well for increased throughput; lower cost per transfection

V-shaped well design for efficient cell recovery

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Configuration: 3-well, 25 µL maximum volume per well
Cell Range: 1x10⁵ - 5x10⁶ per well
Volume Range: 20 - 25 µL per well
Application: Research Use Only
Catalog: SOC-25x3

Graph Details: K562 cells were suspended in MaxCyte^® Electroporation Buffer with pGFP at low concentration (200 µg/mL) or high concentration concentration (400 µg/mL) and transfected in replicates of 2 processing assemblies using low, medium and high electroporation energy. GFP fluorescence was assessed by flow cytometry 24 hours after electroporation.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

R-50x3^TM

Small volume applications and optimization, multi-well

R-50x3™

Research & development applications

Efficient 3 sample processing per transfection

Easy cell recovery with V-shaped well design

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Configuration: 3-well, 55 µL maximum volume per well
Cell Range: 2.25x10⁵ – 1.1x10⁷per well
Volume Range: 45 - 55 µL per well
Application: Research Use Only
Catalog: ER050U3-10

Graph Details: Primary human natural killer (NK) cells were collected from 4 individual donors and activated overnight in 1 ng/mL IL-15. The following day, 3 - 4 x 10⁶ NK cells along with 5 μg mRNA GFP and 45 μL MaxCyte Electroporation Buffer was loaded into each well of the R-50x3 processing assembly and the OC-100 was used as the device control. Cells were electroporated on the MaxCyte GTx™ instrument, then collected and cultured in 1 ng/mL IL-15 media for 24 hours then assessed by flow cytometry. Graphs show the average results and standard error of cells taken from 4 individual donors electroporated with 5 processing assemblies, 2 used for the same donor, with each well of the R-50x3 analyzed individually. The negative control is the average of 4 samples. Experimental data courtesy of Dr. Todd Fehniger’s Laboratory, Washington University in St Louis, Department of Medicine, Division of Oncology with special appreciation to Pamela Wong.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

R-50x8^TM

Small volume discovery applications, multi-well strip

R-50x8™

8-wells for increased throughput; lower cost per transfection

U-shaped well design for efficient cell loading and recovery

Sample tracking with A-H lettering and front writing space

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Configuration: 8-well, 55 µL maximum volume per well
Cell Range: 2.25x10⁵– 1.1x10⁷ per well
Volume Range: 45 - 55 µL per well
Application: Research Use Only
Catalog: ER050U8-03

Graph Details: K562 cells at 5x10⁷ cells/mL were electroporated with plasmid DNA encoding GFP at low energy (Opt-1) and high energy (Opt-10). Four R-50x8 PAs and eight OC-100 PAs were evaluated at each energy level for both GFP expression and cell viability 48 hours post-transfection via flow cytometry analysis. Results are an average of 16 wells for the R-50x8 and 8 wells for the OC-100 for each energy level.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

OC-100^TM RUO

Small volume applications and optimization, single-well

OC-100™ RUO

Small volume applications and experimental optimization

For quick single sample transfection

Well designed for efficient cell loading and recovery

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Configuration: Single well, 100 µL maximum volume
Cell Range: 2.5x10⁵ – 2x10⁷
Volume Range: 50 - 100 µL
Application: Research Use Only
Catalog: SOC-1

Graph Details: K562 cells were suspended in MaxCyte Electroporation Buffer with pGFP at low concentration (200 µg/mL) or high concentration concentration (400 µg/mL) and transfected in replicates of 2 processing assemblies using low, medium and high electroporation energy. GFP fluorescence was assessed by flow cytometry 24 hours after electroporation.

ATx™

For small to medium scale transfection

STx™

For high yield transient expression of complex proteins, vaccines and biologics

OC-100x2^TM

Small volume applications and optimization, multi-well

OC-100x2™

Multi-well for increased throughput; lower cost per transfection

V-shaped well design for efficient cell recovery

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Configuration: 2-well, 100 µL maximum volume per well
Cell Range: 2.5x10⁵ – 2x10⁷ per well
Volume Range: 50 - 100 µL per well
Application: Research Use Only
Catalog: SOC-1x2

Graph Details: K562 cells were suspended in MaxCyte Electroporation Buffer with pGFP into each well of 3 OC-100x2 PAs and 3 OC-100 PAs. Samples were analyzed 24 hours post-electroporation and assayed for fluorescence to measure efficiency. The average for each well is shown in the graph.