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Guide Profiler™

Screening assay

Our in silico Guide Profiler tool enables rapid screening of multiple guide RNAs in parallel, allowing researchers to identify potential off-target effects and narrow down candidates during early discovery and target validation.

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Guide Profiler overview

Guide Profiler

In silico preview of potential off-target effects associated with your gene editing tool. Enables you to start your gene editing risk assessment by rapidly screening sgRNAs to understand the on- and off-target editing risks. Integrates with downstream assays, including Guide Select and ONE-seq for additional cost savings.

In silico evaluation
preview of off-target risk

Rapid turnaround time
Flows seamlessly into nomination assays
Comprehensive report

Rapidly screen candidate guides

Minimize risk from variation at on-target locus
Benchmark against database of guides

Featured resources

A table for guides that then lists off-target score (by MIT and CFD), Activity (CRISPRater), number of loci (L. Distance, and Risk Profiler number.

Poster

Guide Profiler: A Genetic Variant-Aware Computational Tool for Improved Guide RNA Selection for CRISPR-Based Therapeutic Applications

Guide Select nominated off-targets are shown by two tiers (scatter plots) for three guides of interest. The on-target is not presented in this figure.

Example Customer Report

Guide Select Screening Assay Example Report

Poster

ONE-seq for Variant-Aware Therapeutic Guide Selection

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Related screening assays

Guide Select

Off-target assessment assay designed for screening multiple guide RNAs in parallel. Variant-aware searching within a limited search space enables high-powered, low-cost risk assessment for guide RNA selection.