R1000_single

Mid-scale RUO Processing Assemblies

Static electroporation processing assemblies for research applications in mid-volume scales.

Research Use Only (RUO) Processing Assemblies enable the discovery and development of applications at mid-scale. These static electroporation consumables are made from high-grade, inert materials to protect precious cells with additional design features for easy sample loading and recovery.

Choose the right processing assembly for your application

OC-400TM RUO

Mid-scale applications and scale-up optimization, single-well

OC-400 RUO™

Mid-scale applications and scale-up optimization
For quick single sample transfection
Specifically designed for efficient cell loading and recovery

Configuration: Single well, 400 µL maximum volume
Cell Range: 1x106 – 8x107
Volume Range: 200 - 400 µL
Application: Research Use Only
Catalog: SOC-4

OC-400-v-CL-1@2x

Graph Details: To make a master mix, HEK 293 cells were suspended in MaxCyte® Electroporation Buffer at a density of 1x108 cells/mL with pGFP at 200 µg/mL. 400 µL of the master mix was loaded into the OC-400, 1 mL was loaded into one CL-1.1 and 3.5 mL was loaded into a second CL-1.1. Transfection was performed using the MaxCyte HEK electroporation protocol for all 3 PAs. Cells were plated at 3 different densities post-transfection and were analyzed for expression on a flow cytometer after 48 hours.

atx@2x (1)

For small to medium scale transfection

stx@2x (1)

For high yield transient expression of complex proteins, vaccines and biologics

R-1000TM

Mid-scale applications and scale-up optimization, single-well

R-1000™

Mid-scale production applications
Optimization bridge to higher production scales
Efficient and high cell recovery

Configuration: Single-well, 1000 µL maximum volume
Cell Range: 2x106 – 2x108
Volume Range: 400 - 1000 µL
Application: Research Use Only
Catalog: ER001M1-10

Bauer-R-1000-Efficiency-Data-Graphic@2x

Graph Details: KG1a cells were suspended in MaxCyte Electroporation Buffer and mRNA GFP. Electroporation was performed on the MaxCyte GTx™ instrument using the same protocol for all processing assemblies. Final volumes for R-1000, OC-400 and CL-1.1 were 900 μL, 400 μL and 1 mL, respectively. Nine replicates were performed for the R-1000 and OC-400 and three replicates for the CL-1.1, with averages calculated for all graphs.

atx@2x (1)

For small to medium scale transfection

stx@2x (1)

For high yield transient expression of complex proteins, vaccines and biologics

CL-1.1TM RUO

Mid-scale applications and scale-up, closed chamber

CL-1.1™ RUO

Closed chamber with luer locking syringe port
Mid-range production scale
Made from high grade, inert materials to protect precious cells

Configuration: Closed chamber, 3.5 mL maximum volume
Cell Range: 5x106 – 7x108
Volume Range: 1 – 3.5 mL
Application: Research Use Only
Catalog: SCL-1

OC-400-v-CL-1@2x

Graph Details: To make a master mix, HEK 293 cells were suspended in MaxCyte Electroporation Buffer at a density of 1x108 cells/mL with pGFP at 200 µg/mL. 400 µL of the master mix was loaded into the OC-400, 1 mL was loaded into one CL-1.1 and 3.5 mL was loaded into a second CL-1.1. Transfection was performed using the MaxCyte HEK electroporation protocol for all 3 PAs. Cells were plated at 3 different densities post-transfection and were analyzed for fluorescence after 48 hours.

atx@2x (1)

For small to medium scale transfection

stx@2x (1)

For high yield transient expression of complex proteins, vaccines and biologics

Related Reagents and Supporting Products

Related products to consider using with the mid-scale series of Processing Assemblies.

Hyclone-Buffer@2x

Buffer MaxCyte Electroporation Buffer that works with all cell types

PX5

Px5 Workflow Rack Static Processing Assembly rack to assist with workflow management

3.5Kx3@2x

3.5Kx3 Loading Rack CL-1.1 Processing Assembly rack to assist with loading and workflow management

bagkit@2x

Closed Buffer MaxCyte Electroporation Buffer that works with all cell types in a closed process adaptable bag

Need additional information or assistance choosing the right processing assembly?

about-maxcyte@2x