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DEUX-seq™

Nomination assay

SeQure DX's DEUX-seq is a biochemical, orthogonal nomination assay that validates and reinforces ONE-seq™ findings to support regulatory requirements. DEUX-seq leverages in vitro cutting of genomic DNA followed by deep sequencing to determine potential off-target effects with your gene editing tool of interest.

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DEUX-seq overview

DEUX-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased, biochemical, off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.

Nomination

Comprehensive off-target assessment

Highly sensitive
Universal for all editors
Population-scale variant assessment

Off-target assessment
IND filings
Guide RNA selection

Multiple orthogonal methods
Variant effect prediction
GLP

Popular resources

A map of the world with different colored dots representing the varying number of samples across different geographic regions.

Poster

ONE-seq for Variant-Aware Therapeutic Guide Selection

Shown here in a line graph are cumulative counts of candidate off-target loci based on their ONE-seq scores. All loci with assigned ONE-seq scores are represented here. As ONE-seq score increases, number of off-target loci decreases dramatically, indicating that majority of sites do not show clear evidence for cleavage in the ONE-seq assay.

Example Customer Report

ONE-seq Nomination Assay Example Report

A table for guides that then lists off-target score (by MIT and CFD), Activity (CRISPRater), number of loci (L. Distance, and Risk Profiler number.

Poster

Guide Profiler: a genetic variant-aware computational tool for improved guide RNA selection for CRISPR-based therapeutic applications

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Related nomination assays

ONE-seq

Gold standard population-scale, variant-aware, off-target detection. Candidate off-target enumeration, enrichment and biochemical in vitro cutting combined with deep sequencing enables high-sensitivity detection of low-frequency off-target events.

GUIDE-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased, cell-based, off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.