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GUIDE-seq™

Nomination Assay

SeQure Dx's GUIDE-seq is a cell-based, orthogonal nomination assay that validates and reinforces ONE-seq findings to support regulatory requirements. GUIDE-seq utilizes co-transfection of a DNA tag alongside your gene editing tool of interest to profile potential off-target effects in your cell type of interest.

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GUIDE-seq Overview

GUIDE-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased, cell-based off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts. Available either as a fully turn-key assay service provided by SeQure Dx or as a licensable offering.

Nomination

Comprehensive off-target assessment

Highly sensitive
Universal for all editors
Population-scale variant assessment

Off-Target Assessment

Guide RNA Selection

IND Filings

Multiple orthogonal methods
Variant effect prediction
GLP

Featured Resources

Poster

ONE-seq for Variant-Aware Therapeutic Guide Selection

Example Report

ONE-seq Nomination Assay Example Report

Poster

Guide Profiler: a genetic variant-aware computational tool for improved guide RNA selection for CRISPR-based therapeutic applications

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Related Nomination Assays

ONE-seq

Gold-standard population-scale variant-aware off-target detection. Candidate off-target enumeration, enrichment, and biochemical in vitro cutting combined with deep sequencing enables high sensitivity detection of low frequency off-target events.

DEUX-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased biochemical off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.