SeQure On-Target and Off-Target Editing Assessment Services SeQure Comprehensive Suite of On-target and Off-target Assessment Assays SeQure DX GUIDE-seq – Nomination Assay

GUIDE-seq™

Nomination assay

SeQure's GUIDE-seq is a cell-based, orthogonal nomination assay that validates and reinforces ONE-seq™ findings to support regulatory requirements. GUIDE-seq utilizes co-transfection of a DNA tag alongside your gene editing tool of interest to profile potential off-target effects in your chosen cell type.

Request a consultation
Download product sheet
Row herosectionWave Shape Decorative svg added to top

GUIDE-seq overview

SDX-GUIDE-seq Overview

GUIDE-seq

SeQure logos_nomination-icon-text

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased, cell-based, off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts. Available either as a fully turnkey assay service provided by SeQure or as a licensable offering.

Nomination

Comprehensive off-target assessment

  • Highly sensitive​
  • Universal for all editors​
  • Population-scale variant assessment

Off-target assessment​
IND filings
Guide RNA selection

  • Multiple orthogonal methods​
  • Variant effect prediction​
  • GLP

Featured resources

SDX-ONE-seq for Variant-Aware Therapeutic Guide Selection-One-seq incorporates global human genetic diversity
Poster
ONE-seq for Variant-Aware Therapeutic Guide Selection
Shown here in a line graph are cumulative counts of candidate off-target loci based on their ONE-seq scores. All loci with assigned ONE-seq scores are represented here. As ONE-seq score increases, number of off-target loci decreases dramatically, indicating that majority of sites do not show clear evidence for cleavage in the ONE-seq assay.
Example Customer Report
ONE-seq Nomination Assay Example Report
A table for guides that then lists off-target score (by MIT and CFD), Activity (CRISPRater), number of loci (L. Distance, and Risk Profiler number.
Poster
Guide Profiler: A Genetic Variant-Aware Computational Tool for Improved Guide RNA Selection for CRISPR-Based Therapeutic Applications
View all resources
Row seperator3 Shape Decorative svg added to bottom
SeQure logos_nomination-icon

Related nomination assays

ONE-seq

Gold standard population-scale, variant-aware, off-target detection. Candidate off-target enumeration, enrichment and biochemical in vitro cutting combined with deep sequencing enables high-sensitivity detection of low-frequency off-target events. 

DEUX-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased, biochemical, off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts. 

Row seperator3 Shape Decorative svg added to top
Row transparentWave Shape Decorative svg added to top

Learn more about our comprehensive suite of assays spanning from early discovery through pre-clinical and clinical development.

View SeQure's comprehensive suite of on-target and off-target assessment assays
SeQure-Assay Summary_reverse white
Row herosectionWave Shape Decorative svg added to top

Still have questions?

Request a consultation
Young female scientist filling chemical through pipette while working in laboratory