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ONE-seq

Nomination assay

SeQure DX's ONE-seq is the gold standard nomination assay for comprehensive identification of all putative on- and off-target effects for your gene editing tool of interest. ONE-seq leverages variant-aware off-target enumeration coupled with in vitro cutting and deep sequencing to enable precise identification of on- and off-target edits across distinct genetic sub-populations. This high-powered assay offers the most comprehensive risk assessment available for gene editing, ensuring unparalleled confidence in your therapeutic development.

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ONE-seq overview

ONE-seq

Gold standard population-scale, variant-aware, off-target detection. Candidate off-target enumeration, enrichment and biochemical IVC combined with deep sequencing results in high-sensitivity to low-frequency off-target events. Features fully annotated reporting.

Nomination

Comprehensive off-target assessment

Highly sensitive
Universal for all editors
Population-scale variant assessment

Off-target assessment
Guide RNA selection
IND filings

Multiple orthogonal methods
Variant effect prediction
GLP

Popular resources

Visualizations of the three candidate off-target sites with high ONE-seq scores that are present on the same chromosome as the on-target locus. PCSK9-1 has the lowest risk

Poster

ONE-seq for Variant-Aware Therapeutic Guide Selection

Shown here in a line graph are cumulative counts of candidate off-target loci based on their ONE-seq scores. All loci with assigned ONE-seq scores are represented here. As ONE-seq score increases, number of off-target loci decreases dramatically, indicating that majority of sites do not show clear evidence for cleavage in the ONE-seq assay.

Example Customer Report

ONE-seq Nomination Assay Example Report

Circos plot showing rearrangements involving GENE1-1 that were detected in 3/3 replicates. The target chromosome is expanded to facilitate visualization of intra-chromosomal rearrangements. The weight of the lines indicating rearrangements are proportional to log10 of the average total UMI read counts.

Example Customer Report

SAFER Detection Confirmation Assay Example Report

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Related nomination assays

DEUX-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased biochemical off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.

GUIDE-seq

Orthogonal approach to supplement ONE-seq off-target nomination. Unbiased cell-based off-target editing assay provides a complementary approach to supplement your off-target risk assessment efforts.